Pre-Meeting Course

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Pre-Meeting Advanced Flow Cytometry Training Course

In association with Medical University of South Carolina and

The Southeast Flow Cytometry Interest Group

March 28th and 29th, 2017

Cost $375  Please use this link to register:

https://www.eventbrite.com/e/advanced-flow-cytometry-training-tickets-31837611152

 

Course Schedules

Tim Bushnell, Ph.D, Instructor

 Educational Objectives – This two-day course will start by covering the fundamental areas critical for successful flow cytometry:  Reagents, Hardware, Controls, Compensation, and Data Analysis.  The second day will build upon these concepts with a focus on practical application of flow cytometry including: Polychromatic Panel Design, Statistics, Cell Cycle analysis, Apoptosis and Troubleshooting.  At the end of the course, attendee’s will have firm understanding of the principles and how these apply to developing, executing, analyzing and troubleshooting flow cytometry experiments

Target Audience:  Users with basic flow cytometry knowledge seeking to understand the essential concepts in flow cytometry and a desire to move into higher-end assays (from 4 color to 8+ color experiments).  Learning these principles will ensure your flow cytometry experiments will yield results – ultimately saving you time and money in the long run.

Day 1 – Principles of Flow Cytometry

09:00-09:30               Course Introduction and Activity 1 – Design the Experiment

09:30-10:15               Lecture 1 – Software (Reagents, Fluorescence and Fluorochromes)

10:15-10:30               Introduction to Spectral Viewers

10:30-10:45               Coffee Break and questions

10:45-11:30               Lecture 2 – Hardware (Fluidics, Optics and Electronics)

11:30-12:15               Lecture 3 – Compensation Principles and Best Practices

12:15-13:15               Lunch and Sponsor Talk

13:15-14:00               Activity 2 – Compensation practice (w/data)

14:00-14:45               Lecture 4 – Controls in Flow Cytometry

14:45-15:30               Lecture 5 – Understanding Flow Cytometry Data

15:30-15:45               Coffee Break and questions

15:30-16:30               Lecture 6 – Optimization and Standardization in Flow Cytometry

16:30-17:00               Activity 3 – Test yourself

 

 Day 2 – Advanced Topics

 09:00-09:45               Lecture 7 – Theory of Polychromatic Panel Design

09:45-10:30               Activity 4– Panel design

10:30-10:45               Coffee Break and Questions

10:45-11:45               Lecture 8 – Statistics in Flow Cytometry

12:00-13:00               Lunch and Sponsor Talk

13:00-13:45               Lecture 9:  Advanced topic – Cell Cycle Analysis

13:45-14:30               Lecture 10:  Advanced topic – Apoptosis

14:30-15:15               Activity 5 – data analysis

15:15-15:30               Coffee Break and questions

15:30-16:15               Lecture 11:  Advanced topic – CyTOF (beyond 18 colors)

16:15-17:00               Lecture 12: Troubleshooting your data

 

 

Note: a computer with analytical software is helpful but not critical.